Effect of Corbon and Nitrogen Source for the Production of Tetracycline Analysis by Using Hplc
نویسنده
چکیده
Tetracyclines are broad spectrum antibiotic, belong to the family of polyketide antibiotic ,produced by Streptomyces aureofaciens of actinobacteria. The organism Streptomyces aureofaciens 325 was obtained MTCC and cultured on the starch casein media. Screening the organism for the production was confirmed by antibiogram test. The production media was optimized by using carbon sources, and nitrogen sources , Maximum production of antibiotic was shown in soluble starch with 3.0gm concentration tryptophan with the concentration of 0.2gm, and potassium nitrate with 50 mg concentration. The production was increased from 4 day to 7 day and gradually decreased. After the production of tetracycline is quantified by using HPLC.
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تاریخ انتشار 2013